RPA Assay for Chlamydia

Last Update: August 07, 2014

Recently published in The Journal of Molecular Diagnostics, Swedish researchers have developed a 20 minute assay for rapid detection of Chlaymydia trachomatis. The technique uses recombinase polymerase amplification (RPA). The testing was conducted in an Estonian health clinic on 70 patients. There were no false negatives. The RPA Assay works by making many copies of the small circular plasmid DNA of the Chlamydia parasite. After heating of 5 microliters of urine, the DNA sample can be isolated and amplified.

Compared to other existing point-of care tests, the RPA assay has much higher sensitivity (83% vs 10-40%) and higher specificity (100% vs 96%).